Evaluation of the impact of orally administered carbohydrates on postprandial blood glucose levels in different pre-clinical models

Any de Castro Ruiz Marques, Fabiana Percinoto Monteiro Schiavon, Patricia Batista Travassos, Vanessa Fontana Eik, Guilherme Godoy, Christiano Rodrigues Schamber, Roberto Barbosa Bazotte
2016 Brazilian Journal of Pharmaceutical Sciences  
We developed a pre-clinical model in which to evaluate the impact of orally administered carbohydrates on postprandial blood glucose levels. For this purpose, we compared the effects of different carbohydrates with well-established glycemic indexes. We orally administered (gavage) increasing amounts (0.2, 0.4, 0.6, 0.8, and 1.0 g/kg) of sucrose and lactose to rats which had been fasted for 6 h or 15 h, respectively. In part of the experiments we administered frutose (gavagem). Three different
more » ... . Three different models were compared for measuring postprandial blood glucose levels: a) evaluation of interstitial glucose concentrations by using a real time continuous glucose monitoring system; b) evaluation of glucose levels in blood obtained from the rat tail; c) evaluation of serum glucose levels in blood collected after decapitation. Our results showed that blood obtained from the tails of 15-h fasted rats was the best model in which to evaluate the effect of carbohydrates on postprandial blood glucose levels.
doi:10.1590/s1984-82502016000400021 fatcat:jlwfi7d7jfctlm26eymc35hmsq