Method for Estimation of Toxic Endotoxin in Inactivated Salmonella Vaccine in D-Galactosamine-Sensitized Mice

Noriyasu TAKIKAWA, Kazuyoshi KAWAHARA, Akira SAWATA, Katsumi KUME
2004 Journal of Veterinary Medical Science  
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosaminesensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD 50 in the
more » ... e LD 50 in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
doi:10.1292/jvms.66.1591 pmid:15644614 fatcat:wxz3a5y6yzbvtdrdbrdtvjqxbm