Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais

Vitor Cardoso Aragão, Andréia Anschau, Barbara Daniele Almeida Porciuncula, Cleidi Thiesen, Susana Juliano Kalil, Carlos André Veiga Burkert, Janaína Fernandes de Medeiros Burkert
2009 Química Nova  
Recebido em 28/5/08; aceito em 21/5/09; publicado na web em 10/11/09 ENZYMATIC SYNTHESIS ISOAMYL BUTYRATE EMPLOYING COMMERCIAL MICROBIAL LIPASES. Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solventfree system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water
more » ... amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L -1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.
doi:10.1590/s0100-40422009000900005 fatcat:wowpmgc6qjdbtewfea5bd2y6rm