A Novel Molecularly Imprinted Polymer for the Solid-Phase Extraction of Tanshinones from Serum

Hongliang He, Hong Li, Yankun Gao, Dong Chen, Liying Shi, Jin Peng, Shuhu Du, Lina Chen
2014 Analytical Letters  
A restricted access molecularly imprinted polymer (RAMIP) crosslinked with bovine serum albumin (BSA) was prepared on the surface of the mesoporous UiO-66-NH 2 metal-organic framework (MOF). The surface morphology, imprinting behavior, and protein exclusion properties of UiO-66-NH 2 @RAMIP@BSA were investigated. The maximum adsorption capacity was 50.55 mg g À1 for ofloxacin, with a 99.4% protein exclusion rate. Adsorption equilibrium was reached in 9 min. Combined with RP-HPLC, a solidphase
more » ... LC, a solidphase extraction column filled with UiO-66-NH 2 @RAMIP@BSA was used to selectively enrich and analyze ofloxacin and enrofloxacin antibiotics from bovine serum with recoveries of 93.7-104.2% with relative standard deviations of 2.0-4.5% (n ¼ 3). The linear range and the limit of detection were 0.1-100 mg mL À1 and 15.6 ng mL À1 , respectively. These results suggest that UiO-66-NH 2 @RAMIP@BSA is an efficient pretreatment adsorbent for biological sample analysis.
doi:10.1080/00032719.2014.930865 fatcat:w3uii7lcivfupok6r65ahvupym