Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases

Thomas Strong, Scot Dowd, Alexander F. Gutierrez, David Molnar, Jonathan Coffman
2013 F1000Research  
Discuss this article (0) Comments 3 2 1 SHORT RESEARCH ARTICLE Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases [version 2; referees: 3 approved with reservations] Previously titled: Amplicon pyrosequencing of wild duck eubacterial microbiome from a fecal sample reveals numerous species linked to human and animal diseases Abstract Our investigation into the composition of the
more » ... d duck, , Aythya americana eubacterial microbiome from a fecal sample using amplicon pyrosequencing revealed that the representative bacterial species were quite distinct from a pond water sample, and we were able to classify the major operational taxonomic units with , , Fusobacterium mortiferum Streptobacillus moniliformis , , Lactobacillus intermedius Actinomyces suimastitidis Campylobacter , , , ., Canadensis Enterococcus cecorum Lactobacillus aviarus Actimomyces spp . representing the majority of Pseudobutyrivibrio spp and Helicobacter brantae the eubacterial fecal microbiome. Bacterial species present in the analysis revealed numerous organisms linked to human and animal diseases including septicemia, rat bite fever, pig mastitis, endocarditis, malar masses, genital infections, skin lesions, peritonitis, wound infections, septic arthritis, urocystitis, gastroenteritis and drinking water diseases. In addition, to being known carriers of viral pathogens wild ducks should also be recognized as a potential source of a range of bacterial diseases. How to cite this article: et al. Amplicon pyrosequencing and ion torrent sequencing of wild duck eubacterial microbiome from fecal samples reveals numerous species linked to human and animal diseases [version 2; referees: 3 2013, :224 (doi: ) approved with reservations] F1000Research 2 PubMed Abstract | Publisher Full Text 3. Bahl J, Krauss S, Kuhnert D, et al.: Influenza a virus migration and persistence in north american wild birds. PLoS Pathog. 2013; 9(8): e1003570. PubMed Abstract | Publisher Full Text | Free Full Text 4. Kocer ZA, Krauss S, Stallknecht DE, et al.: The potential of avian H1N1 influenza A viruses to replicate and cause disease in mammalian models. PLoS One. 2012; 7(7): e41609. PubMed Abstract | Publisher Full Text | Free Full Text 5. 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doi:10.12688/f1000research.2-224.v2 fatcat:d2nyozfl2ffznkao5dpypdyrc4