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Pakistan Veterinary Journal
In the present report, a new assay combining specific substrates and a selective BChE inhibitor (ethopropazine hydrochloride) was used to measure both AChE and BChE in canine whole blood samples. Acetylthiocholine iodide (ATCI) and butyrylthiocholine iodide (BTCI) were used as substrates, whereas 2,2'-dithiodipiridine was used as chromophore. Ethopropazine concentration inhibiting over 95% BChE with minimum AChE inhibition was fixed at 0.3mM. The results confirmed that whole bloodfatcat:5xgrgtqkb5dm5gw6ijbfbfsq3y