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Two-photon excited fluorescence microscopy (two-photon microscopy), a technology based on multi-photon excitation induced by an ultra-short pulse near infrared laser light, is one of the most promising candidates for functional analysis of cells and biomolecules in intact living organs or tissues. Two-photon microscopy facilitates the investigation of the molecular basis and the pathology underlying exocytosis. This review describes both the current and anticipated capabilities of two-photondoi:10.5360/membrane.35.57 fatcat:rmtkkt3ryvb4vfh4fdpu3l476i