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The software was evaluated on images both acquired by us and by the Hao laboratory  . The performance in segmentation was evaluated on three datasets and in tracking on four datasets. One dataset for both area and tracking was acquired from , and the remaining datasets were acquired by us. For the images acquired in our lab, we used the following culture conditions. The cells in the experiment were plated on 2% XY Dextrose plates, inoculated in synthetic complete (SC) media at a very lowdoi:10.1093/bioinformatics/btx550 pmid:28968663 fatcat:mns4rrrbb5g4tbkdzvenbidxbm