Morphologically constrained and data informed cell segmentation of budding yeast

Elco Bakker, Peter S Swain, Matthew M Crane, Robert Murphy
2017 Bioinformatics  
The software was evaluated on images both acquired by us and by the Hao laboratory [7] . The performance in segmentation was evaluated on three datasets and in tracking on four datasets. One dataset for both area and tracking was acquired from [7], and the remaining datasets were acquired by us. For the images acquired in our lab, we used the following culture conditions. The cells in the experiment were plated on 2% XY Dextrose plates, inoculated in synthetic complete (SC) media at a very low
more » ... tarting concentration and loaded directly into the microfluidic device after 24 hours while still in exponential growth. A range of strains, imaging conditions and growth media were used to reflect the range of experiments undertaken in the laboratory (Table 1) .
doi:10.1093/bioinformatics/btx550 pmid:28968663 fatcat:mns4rrrbb5g4tbkdzvenbidxbm