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Comparison of human sperm motility, morphology, and sperm membrane integrity using two cryoprotective media, Test-yolk buffer with glycerol and glycerol alone. Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Semen characteristics were evaluated before freezing and after thawing. Motility was measured at 0, 60, 120, and 180 minutes post-thaw following removal of the cryoprotectant (post-wash). Percentage ofpmid:10721743 fatcat:guhhv2zawvb67fkx3awhbmhima