Articles of Significant Interest Selected from This Issue by the Editors

2009 Journal of Virology  
Functions of Human Cytomegalovirus gB Phenotypic complementation of mutant viruses with deletions in essential genes has been routine for most herpesviruses for many years. However, this strategy has not been possible for human cytomegalovirus (HCMV) essential glycoproteins due to the restricted cellular tropism and long replication cycle of this virus. Using retroviral transduction, Isaacson and Compton (p. 3891-3903) have succeeded in propagating a glycoprotein B (gB) deletion HCMV mutant.
more » ... ion HCMV mutant. Analysis of this virus shows that HCMV gB is required for virus-cell and cell-cell fusion but dispensable for attachment, assembly, and egress. This new genetic system will now allow for detailed structure-function analyses of other essential HCMV gene products.
doi:10.1128/jvi.00366-09 fatcat:cn7cjljph5djrcgwm6ro6y67xm