Optimization of culture conditions of Bacillus subtilis with α-glucosidase inhibitory activity

Yong-Soon Kim, Wan-Taek Ju, Hyun-Bok Kim, Gyoo-Byung Sung
2016 International Journal of Industrial Entomology  
1-Deoxynojirimycin (DNJ) have been extensively investigated for their α-glucosidase inhibitor on postprandial hyperglycemia, and applied in nutraceuticals and medicine for preventing or delaying progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (about 0.1%), therefore, more effective extraction method is needed. This study was performed to develop microbial DNJ for biological methods of DNJ as an alternative to the chemical methods. In this study, we obtained
more » ... vidence for Bacillus subtilis that produce DNJ in large quantities by high performance liquid chromatography. Inhibition of α-glucosidase activity was determined to DNJ production or non-production. Investigation of the effect of mulberry leaves powder concentration (1~5%), using the DNJ high-production bacteria, provided evidence for microbial mass production of DNJ. When the 4% mulberry leaf powder for 9 days was used, the α-glucosidase inhibitory activity was over the 85%. Also, the results presented in this study confirm DNJ yield's increasement in microbes using the various of nutrients and provide insight of ways to improve DNJ yields in microorganisms. few days, ground to powder by a motar and pestle, and passed through 150 μm sieves.
doi:10.7852/ijie.2016.33.1.24 fatcat:pjeyjxo525bhripmjzbrldqbxm