Excessive and non-specific use of broadspectrum antibiotics has converted Enterococci from a normal gut commensal to opportunistic nosocomial as well as community acquired pathogen. The high level resistance to Aminoglycosides (HLAR) is long known and constantly increasing problem. Concomitant Vancomycin resistance (VRE) reduces the treatment options and increases potential risk of Vancomycin resistant gene transfer to Staphylococcus aureus. Aim: To study the antibiogram of Enterococcus

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... at a tertiary care teaching hospital. Method: A retrospective analysis was performed in microbiology laboratory of a tertiary care teaching hospital in Durg. All the Enterococcus isolated from urine, pus, blood and body fluids received between January, 2016 and June, 2017, were included in the study. The demographic details of patients were noted. All the isolates were identified and speciated by standard microbiologial procedures. Antimicrobial susceptibility was tested for all the isolates by modified Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines (2015). Results: Among 178 Enterococcal species that were isolated and biochemically identified, 56% were Enterococcus faecalis, 40% were E. faecium and 4% were other species. Out of 178 isolates, most (60%) were from female patients. Most of the isolates (62%) were from urine followed by pus (25%), blood and others. Penicillin resistance and HLAR were seen in 50 and 58%, respectively. Three urinary isolates, all identified as E. faecium, were found to be resistant to Vancomycin by disk diffusion method. Among the urinary isolates, Nitrofurantoin resistance was seen in only 4.5% samples. Conclusion: VRE were found to be rare in this area, but Penicillin resistance and HLAR were quite high. This may lead to increased use of higher antibiotics like Vancomycin and Linezolid, leading to increased selective pressure of these antibiotics to form resistance in near future.