Monoclonal Antibodies Recognizing Structural Components of Murine Retroviruses Including an FMR Antigen on Protein p12

C. Gambke, H. P. Senn, G. Hunsmann, G. Schumann, C. Moroni, S. S. Alkan
1984 Journal of General Virology  
Monoclonal antibodies were prepared from mice and rats immunized with Friend leukaemia virus and BALB/c xenotropic virus. By immunoprecipitation of ~zsIlabelled and [35S]methionine-labelled viruses and by protein blotting, ten antibodies were found to react with the viral components p12, p15, p30, gp70 and pl5E/pl2E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukaemia viruses (MuLVs). When tested on a panel of ecotropic and
more » ... el of ecotropic and xenotropic MuLVs the antibodies revealed the following antigenic specificities: ecotrop-specific on pl5E/p12E; xenotrop-specific on pl5E; groupspecific on p30 and pl5E; FM-specific on gp70; FR-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing an FMR determinant localized on p12.
doi:10.1099/0022-1317-65-9-1507 pmid:6206201 fatcat:7gqzx7i6zrahtb27yfugijathu