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Endophytic fungi associated with Chinese medicinal plant Dendrobium officinale have been revealed using the culture-dependent approach and the diversity and antimicrobial activity have been examined in this study. A total of 105 endophytic isolates representing 16 fungal morphotypes were recovered from the roots, stems and leaves of D. officinale. Identification by ITS-rDNA sequencing showed 12 distinct operational taxonomic units, and most of them were classified into species level. Shannon'sdoi:10.18520/cs/v112/i08/1690-1697 fatcat:e55mkpr7ajg5rhidykyyxkiyqq