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Formation of Pseudomonas aeruginosa inhibition zone during tobramycin disk diffusion is due to transition from planktonic to biofilm mode of growth
2019
International Journal of Antimicrobial Agents
Pseudomonas aeruginosa PAO1 (tobramycin MIC = 0.064 μg/mL) was used to perform agar diffusion tests employing tobramycin-containing tablets. Bacterial growth and formation of inhibition zones were studied by stereomicroscopy and by blotting with microscope slides and staining with methylene blue, Alcian blue and a fluorescent lectin for the P. aeruginosa PSL, which was studied by confocal laser scanning microscopy. Diffusion of tobramycin from the deposit was modelled using a 3D geometric
doi:10.1016/j.ijantimicag.2018.12.015
fatcat:taopd2uz5ndjngdbnnefunyjj4