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Use of the Polymerase Chain Reaction to Detect Legionella DNA in Urine and Serum Samples from Patients with Pneumonia
1996
Clinical Infectious Diseases
Legionella pneumonia can be difficult to diagnose. Existing laboratory tests for detecting Legionella species lack sensitivity or provide only a retrospective diagnosis. We used the polymerase chain reaction (PCR) with primers that amplify a 104-base pair segment of the coding region of the SS rRNA gene to detect Legionella DNA in urine and serum samples from patients with pneumonia. Stored urine and serum samples from patients enrolled in two prospective studies of pneumonia were tested.
doi:10.1093/clinids/23.3.475
pmid:8879767
fatcat:fixtk7ebxrazznqb74q7p2crym