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The use of agricultural residues as a substrate is a promising option for orchid acclimatization, which is a critical stage of micropropagation. Thus, this study aimed to evaluate the use of grape residue S-10 Beifort ®, isolated or mixed with other agricultural residues, in the acclimatization of Oncidium baueri Lindl. Five treatments were studied (S-10 Beifort ®; S-10 Beifort® + carbonized rice husk (CRH); S-10 Beifort® + coconut fiber (CF); S-10 Beifort® + CRH + CF; CRH + CF), with fourdoi:10.1590/2447-536x.v28i2.2477 doaj:4e12c63dd465447f82c56ef3ac798143 fatcat:fsaczelex5fuzpjx7rwbva6j6a