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Withania somnifera (L) Dunal.var.JA20 (Jawahar Asgund 20), JA134 (Jawahar Asgund134), GLV(Gujarat Local Variety) and wild plants were regenerated from callus cultures on MS medium supplemented with BAP and 2,4-D. DNA finger printing by PCR method with randomly amplified primers was carried out for mother plants and regenerated plants. Of 10 different primers from OPBE series, 3 primers namely OPBE 09-GGAAGCGTCC, OPBE 15-CTTTGCGCAC and OPBE 18-GGGAAAAGCC produced polymorphisms. Minor variationfatcat:niirskhvhvcn3dxjrcmmmq64ve