ANALYSIS OF THE VARIANTS PRODUCED THROUGH TISSUE CULTURE TECHNIQUES IN WITHANIA SOMNIFERA (L) DUNAL. BY DNA FINGER PRINTING EMPLOYING RAPD METHOD

Devika Shetty‫٭‬, Nareshchandra
unpublished
Withania somnifera (L) Dunal.var.JA20 (Jawahar Asgund 20), JA134 (Jawahar Asgund134), GLV(Gujarat Local Variety) and wild plants were regenerated from callus cultures on MS medium supplemented with BAP and 2,4-D. DNA finger printing by PCR method with randomly amplified primers was carried out for mother plants and regenerated plants. Of 10 different primers from OPBE series, 3 primers namely OPBE 09-GGAAGCGTCC, OPBE 15-CTTTGCGCAC and OPBE 18-GGGAAAAGCC produced polymorphisms. Minor variation
more » ... s observed between mother plants and regenerants of W.somnifera (L) Dunal var.JA20, JA134 and GLV. The regenerated and mother plants of JA20 variety showed minor variation in the first band and third band. Regenerated and mother plants of JA134 variety showed variation between first bands. GLV depicts variation between 3 rd , 4 th and 5 th bands and similarity was observed between 1 st and 2 nd bands. There was no variation between regenerants and mother plants of wild plants selected for the present investigation.
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