Application of silica extracted from rice husk ash for the encapsulation of AFB1 antibody as a matrix in immunoaffinity columns

Deni Pranowo, Nuryono, Ali Agus, Jumina, Romsyah Maryam, FMCS Setyabudi
2017 JSM Mycotoxins  
The extraction of silica from rice husk ash (RHA) for the encapsulation of aflatoxin B 1 antibody (Ab-AFB 1 ) and its application as a matrix in immunoaffinity columns (IACs) were achieved. The RHA extraction was performed using 4 M NaOH, which yielded sodium silicate (Na 2 SiO 3 ) for the synthesis of silica gel. The obtained silica was used for encapsulating Ab-AFB 1 using the sol-gel technique. One milliliter of 1 M Na 2 SiO 3 :H 2 O:H 3 PO 4 (0.43:0.11: 0.46) could generate silica gel that
more » ... as suitable for encapsulating 1.36 mg of Ab-AFB 1 at pH 7. After 48 hours of aging, the silica gel modified with Ab-AFB 1 (SG-Ab-AFB 1 ) was ground, and packed as the matrix in the IAC for aflatoxins purification. The modified silica gel was characterized using FTIR and SEM. The properties of IAC with SG-Ab-AFB 1 were investigated by evaluating AF recovery, binding capacity, and reusability. The recovery of AFB 1 was 94.11 ± 4.62%. In addition to AFB 1 recovery, the column also retained AFB 2 , AFG 1 , and AFG 2 with recovery values of 98.22 ± 3.74%, 92.22 ± 7.62%, and 83.00 ± 6.31%, respectively. This column, which contained 0.5 g of SG-Ab-AFB 1 had a binding capacity of approximately 50 ng of AFs per column, and could be reused at least 5 times with a recovery of more than 80%.
doi:10.2520/myco.67_2_1 fatcat:en3fiuqxdrehbfxnohvq7dy3um