Original Article CD38 plays key roles in both antioxidation and cell survival of H 2 O 2-treated primary rodent astrocytes

Yingxin Ma, Danhong Wu, Xianting Ding, Weihai Ying
2014 Int J Physiol Pathophysiol Pharmacol   unpublished
CD38 is an ecto-enzyme that consumes NAD + to produce cyclic ADP-ribose (cADPR)-a potent agonist of ryanodine receptors. Recent studies have suggested CD38 may play significant roles in both ischemic brain injury and traumatic brain injury, while the mechanisms underlying the roles of CD38 in neurological diseases remain unclear. Because oxidative stress plays key roles in both ischemic brain damage and traumatic brain damage, in this study we used primary astrocyte cultures as a experimental
more » ... as a experimental model to test our hypothesis that CD38 may play significant roles in oxidative stress-induced neural cell death. We found CD38 siRNA-produced decrease in CD38 levels can lead to a significant increase in H 2 O 2-induced astrocyte death. Moreover, the CD38 siRNA treatment can significantly aggravate oxidative stress in the H 2 O 2-treated cells, as indicated by increases in both superoxide and lipid peroxidation, suggesting that CD38 is required for maintaining the antioxidation capacity of the cells. We also found that H 2 O 2 can induce increased CD38 expression. Collectively, our study has obtained novel findings suggesting that CD38 plays a significant role in both antioxidation and cell survival of reactive oxygen species-exposed primary astrocytes, suggesting that CD38 may become a novel target for decreasing the oxidative damage in neu-rological disorders.