TNF -Mediated Inhibition and Reversal of Adipocyte Differentiation Is Accompanied by Suppressed Expression of PPAR without Effects on Pref-1 Expression

H. Xing
1997 Endocrinology  
Tumor necrosis factor ␣ (TNF␣) is a polypeptide hormone with pleiotropic effects on cellular proliferation and differentiation. To investigate how TNF␣ inhibits and reverses adipocyte differentiation, we studied the expression of two factors involved in the adipocyte differentiation process. Peroxisome proliferator-activated receptor ␥ (PPAR␥) is a positive regulator of adipogenesis, whereas preadipocyte factor 1 (Pref-1) inhibits adipocyte differentiation. The expression patterns of both PPAR␥
more » ... and Pref-1 change during early stages of adipocyte differentiation. Decreased expression of Pref-1 and increased expression of PPAR␥ occur 1 day and 2 days, respectively, after 3T3-L1 cells reach confluence. During TNF␣-mediated inhibition of adipocyte differentiation, PPAR␥ messenger RNA (mRNA) expression stays at low levels. In contrast, TNF␣ treatment has no effect on the normal decrease in Pref-1 gene expression that occurs during adipogenesis. We observed that certain cytokine and growth
doi:10.1210/en.138.7.2776 pmid:9202217 fatcat:4fm4s4fpkzbcpc4ctt7i2uhmte