Calcium dependent astrocyte-neuron communication

Charles Edward Alexander Stringer
The discovery of direct communication between astrocytes and neurons has changed the perception of astrocytes from passive support cells to active partners in information processing. Astrocytes express myriad neurotransmitter receptors and have been shown to release neurotransmitters, allowing these cells to respond and signal to adjacent neurons, respectively. Astrocytes can rapidly respond to neurotransmitters with a rise in [Ca²⁺]i and astrocyte neurotransmitter release has been shown to be
more » ... alcium dependent. The purpose of this research was to investigate unknown aspects of astrocyteneuron communication with in situ calcium imaging to improve our understanding of how these cells interact functionally. The first objective of this research was to determine whether extracellular dopamine elicits astrocyte calcium transients in the prefrontal cortex (PFC). Astrocytes from this area express dopamine receptors and dopamine is an important neurotransmitter in the PFC. We found that astrocytes in PFC brain slices reliably respond to a high concentration of dopamine ([50μM]) with [Ca²⁺]i transients, however these responses were due to the activation of adrenoreceptors, not dopamine receptors. The inability of a lower concentration of dopamine ([10μM]) to elicit astrocyte [Ca²⁺]i transients questions the whether these cells can rapidly respond to PFC dopamine at physiological levels. The second objective of this research was to investigate the extent that calcium dependent glutamate release from astrocytes is able to influence neural activity. The best studied mechanism of astrocyte gliotransmitter release is the calcium dependent release of glutamate which has been demonstrated in single astrocytes in situ. We used the vasoactive peptide endothelin to preferentially elicit widespread astrocyte [Ca²⁺]i transients astrocytes from hippocampal brain slices to determine whether the widespread calcium rise in astrocytes was associated with a change in glutamate sensitive synaptic transmission. Despit [...]
doi:10.14288/1.0101300 fatcat:lkaasfjcdraltg33ojegf47nl4