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Sequence analysis of the nicks and termini of bacteriophage T5 DNA
Journal of Virology
Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [y-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [a-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least thedoi:10.1128/jvi.22.2.520-526.1977 fatcat:dsgj7jfqdbfs3f63xmicocqqxe