Sequence analysis of the nicks and termini of bacteriophage T5 DNA

B P Nichols, J E Donelson
1977 Journal of Virology  
Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [y-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [a-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the
more » ... nce -PUOH pGpCpGpCin common. In addition, the two 5' external termini had the first seven nucleotides in common. on May 9, 2020 by guest
doi:10.1128/jvi.22.2.520-526.1977 fatcat:dsgj7jfqdbfs3f63xmicocqqxe