Helicobacter pylori HP0231 Influences Bacterial Virulence and Is Essential for Gastric Colonization

Yu Zhong, Florian Anderl, Tobias Kruse, Franziska Schindele, Elżbieta Katarzyna Jagusztyn-Krynicka, Wolfgang Fischer, Markus Gerhard, Raquel Mejías-Luque, Ivo G. Boneca
2016 PLoS ONE  
The Dsb protein family is responsible for introducing disulfide bonds into nascent proteins in prokaryotes, stabilizing the structure of many proteins. Helicobacter pylori HP0231 is a Dsb-like protein, shown to catalyze disulfide bond formation and to participate in redox homeostasis. Notably, many H. pylori virulence factors are stabilized by the formation of disulfide bonds. By employing H. pylori HP0231 deficient strains we analyzed the effect of lack of this bacterial protein on the
more » ... ality of virulence factors containing putative disulfide bonds. The lack of H. pylori HP0231 impaired CagA translocation into gastric epithelial cells and reduced VacA-induced cellular vacuolation. Moreover, H. pylori HP0231 deficient bacteria were not able to colonize the gastric mucosa of mice, probably due to compromised motility. Together, our data demonstrate an essential function for H. pylori HP0231 in gastric colonization and proper function of bacterial virulence factors related to gastric pathology.
doi:10.1371/journal.pone.0154643 pmid:27138472 pmcid:PMC4854439 fatcat:yb7s4mp5mzampozoazdu6gnr2e