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Acetohydroxyacid synthase (Ec 22.214.171.124) catalyses the thiamine diphosphate-dependent reaction between two molecules of pyruvate yielding 2-acetolactacte and CO 2 . The enzyme will also utilise hydroxypyruvate with a k cat value that is 12% of that observed with pyruvate. When hydroxypyruvate is the substrate, the enzyme undergoes progressive inactivation with kinetics that are characteristic of suicide inhibition. It is proposed that the dihydroxyethyl-thiamine diphosphate intermediate can expeldoi:10.1080/14756360400020553 pmid:15895677 fatcat:xekwvqqhfbek5hrkt4bxscoqda