Histopathological and Behavioral Characterization of a Novel Cervical Spinal Cord Displacement Contusion Injury in the Rat

D.D. Pearse, T.P. Lo, K.S. Cho, M.P. Lynch, M.S. Garg, A.E. Marcillo, A.R. Sanchez, Y. Cruz, W.D. Dietrich
2005 Journal of Neurotrauma  
Models of spinal cord injury (SCI) caused by weight-drop devices to cause contusion have been used extensively, and transient behavioral deficits after thoracic injury have been demonstrated. The severity of the injury caused by the device should be mild enough to allow recovery. Objective: To determine whether our adapted weight-drop device with a small tip can effectively induce mild hemicontusion at the level of the fifth cervical vertebra. Methods: We divided 15 adult male Sprague Dawley
more » ... e Sprague Dawley rats into groups of 5 for the following treatments: sham (SH, laminectomy only), mild (MSCI) or severe SCI (SSCI). Behavioral tests and histopathology were used before (day 1) and after the treatment on days 3, 7, 14, 21, 28, and 35 to assess the injury. Results: Rats with SSCI showed a significant somatosensory deficit on days 3 and 7 compared with rats in the SH group, recovering by day 14. In a horizontal-ladder test of skilled locomotion, rats with SSCI showed a significant increase in error scores and percentage of total rungs used, and a decrease in the percentage of correct paw placement compared with rats in the SH group. There was greater recovery to normal paw placement by rats with MSCI than by rats with SSCI. These behavioral deficits were consistent with histopathology using hematoxylin and eosin counterstained Luxol fast blue, indicating the degree of injury and lesion area. Conclusions: Mild hemicontusion caused by the adapted device can be used to evaluate SCI and provides a model with which to test the efficacy of translational therapies for SCI. Animal models of spinal cord injury (SCI) using contusion have been developed to mimic signs and symptoms similar to those seen after SCI in humans [1] because most SCIs in humans involve spinal cord contusion. Histopathology in these models show an intact dura and external aspects of the spinal cord, but an internal cystic cavity. The loss of motor Unauthentifiziert | Heruntergeladen 06.03.20 05:39 UTC
doi:10.1089/neu.2005.22.680 pmid:15941377 fatcat:pfmravswa5hipgrlvvicrma3am