Fowl semen was diluted :% and stored at o°to 2°C for 24 and 48 hr before insemination. The diluents were two modifications of a glutamate-containing saline solution calculated to have an osmotic pressure equivalent to 0-98 to 1 % sodium-chloride solution. The concentrations of sodium, potassium, magnesium and glutamate in the diluents were of the same order as those found in seminal plasma uncontaminated with cloacal gland fluid. Calcium was not added and the chloride concentration was low

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... red with undiluted seminal plasma. One modification of the diluent contained added fructose and the other was fructose-free. Pullets were inseminated with o-i ml of the diluted semen. The proportion of fertile eggs laid by all pullets during days 2 to 6 following insemination with semen stored for 24 and 48 hr in the solution con¬ taining fructose was 64 and 47 %, respectively. Fertility was significantly lower in pullets inseminated with semen stored in the fructose-free solution.