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Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
2011
Nucleic Acids Research
Standard Illumina mate-paired libraries are constructed from 3-to 5-kb DNA fragments by a blunt-end circularization. Sequencing reads that pass through the junction of the two joined ends of a 3-5-kb DNA fragment are not easy to identify and pose problems during mapping and de novo assembly. Longer read lengths increase the possibility that a read will cross the junction. To solve this problem, we developed a mate-paired protocol for use with Illumina sequencing technology that uses Cre-Lox
doi:10.1093/nar/gkr1000
pmid:22127871
pmcid:PMC3273786
fatcat:iscv6i2h7rgl3f35hvtpp7fuxm