Comparison Study of Molecular Diagnostic Reagents for COVID-19 Pooling Test

Dong Hee Seo, Geun-ju Son, Jeong-woo Kang
2021 Journal of Laboratory Medicine and Quality Assurance  
In Korea, real-time reverse transcription-polymerase chain reaction (PCR) is performed on individual or pooled samples to diagnosis coronavirus disease 2019 (COVID-19). New developed LabGun COVID-19 ExoFast RT-PCR Kit (LabGenomics, Korea) was compared to PowerCheck SARS-CoV-2 Real-time PCR Kit (KogeneBiotech, Korea) for its properness about COVID-19 pooling test. LabGun kit showed 98.75% concordance for positive samples and 100% concordance for negative samples tested by PowerCheck kit. Agarose
more » ... gel electrophoresis study revealed similar PCR products band intensities for two testing kits. In conclusion, LabGun kit could be used for COVID-19 pooling test.
doi:10.15263/jlmqa.2021.43.4.214 fatcat:7iph6ol4rjhy5oryieucbpfvya