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Comparison of The Variability of Small Extracellular Vesicles Derived From Human Liver Cancer Tissues And Cultured From The Tissue Explants Based On A Simple Enrichment Method: Yield, Purity And Molecular Markers [post]

Jie Chen, Zhigang Jiao, Jianwen Mo, Defa Huang, Zhengzhe Li, Wenjuan Zhang, Tong Yang, Minghong Zhao, Fangfang Xie, Die Hu, Xiaoxing Wang, Xiaomei Yi (+2 others)
2021 unpublished

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A potential of using small extracellular vesicle (sEV) for diagnostic and therapeutic purposes has attracted great interest. Some sEVs, directly derived from various tissues, can reflect the physiological and pathological state of the organism. Currently, there are two sources for obtaining tissue-derived sEV: the interstitial space of tissues and cultivation of tissue explants. The former was obtained from tissues that were digested with enzymes and the latter was released by tissue explants.
more » ... n the present study, we established a new method for the isolation and purification of sEVs from the interstitial space of human liver cancer tissue. Tissues-derived sEVs (TdsEVs) isolated by this method and cultured explants-derived sEVs (cedsEVs) were both characterized by nano-flow cytometry (NanoFCM) for concentration, size distribution and purity. These vesicles were identified and compared by transmission electron microscopy and western blot. TdsEVs have a larger particle size, higher particle concentration and purity than cedsEVs. This study establishes a simple sEV isolation and purification protocol and provides a basis for selection and reference for researches of tdsEVs and cedsEVs.
doi:10.21203/rs.3.rs-680243/v1 fatcat:ij6l2xvdinenboeayvh7fdpbay
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Jie Chen, Zhigang Jiao, Jianwen Mo, Defa Huang, Zhengzhe Li, Wenjuan Zhang, Tong Yang, Minghong Zhao, Fangfang Xie, Die Hu, Xiaoxing Wang, Xiaomei Yi, Yu Jiang, Tianyu Zhong. "Comparison of The Variability of Small Extracellular Vesicles Derived From Human Liver Cancer Tissues And Cultured From The Tissue Explants Based On A Simple Enrichment Method: Yield, Purity And Molecular Markers." (2021)