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Nanoscopy with focused light For more than a century, it has been widely accepted that diffraction of light precludes any lens-based optical microscope from discerning details smaller than about half of the wavelength of light (~200 nm). However, in the 1990's it was discovered that basic state transitions in a fluorophore can be exploited to eliminate the resolution-limiting role of diffraction. Since then, fluorescence microscopes have been developed that are now able to resolve on thedoi:10.1126/science.1137395 pmid:17525330 fatcat:vjn3abvopfesdguj4g6vsnlb7e