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EXPERIMENTAL PROCEDURES Cell Culture and Transfection-A human keratinocyte cell line (Ha-Cat), human lung epithelial cell line (WI-38), simian kidney epithelial cell line (COS-1), rat osteosarcoma cell lines (UMR-106 and UMR-108), and human osteosarcoma cell lines (U2-OS and SAOS) were cultured in Dulbecco's modified Eagle's medium (Cellgro) supplemented with 10% fetal calf serum (HyClone), 50 units/ml penicillin, and 50 g/ml streptomycin (BioWhittaker). HeLa cells were cultured in Dulbecco'sdoi:10.1074/jbc.m204553200 pmid:12105216 fatcat:zkkzcblbpnefhavsiqi5xotcty