Development of A First-Responder Fluorescence Reader for Microarray Cytokine Assay of Human Immune Response to Disease

David B. Fenner, David I Rosen, Anthony A Ferrante, Amy E Stevens, Chad E Bigelow, Steven J Davis
2007 Materials Research Society Symposium Proceedings  
Development of field portable apparatus and methods for cytokine assay of human saliva by fluorescent-reporting microarray plates is described. Multiplexed assay of 12 cytokines for minimally-processed saliva is read with a CCD-based imager under LED excitation. Immune responsive cytokines are measured at levels significant for indication of human disease state. The motivational context for the new apparatus development, the general optical design issues, saliva protocol, and image analysis are
more » ... image analysis are described.
doi:10.1557/proc-1004-p03-26 fatcat:47ln7fz3iffwjjc4lijo4ob7gq