Localization of Escherichia coli RNA polymerase binding sites on bacteriophage S13 and 0X174 DNAs by electron microscopy

E Rassart, J H Spencer, M Zollinger
1979 Journal of Virology  
genomes. The major locations on S13 have been mapped at 8 to 10 and 92 to 96% of the genome length, starting from the unique Pst I cleavage site. The locations correspond to the beginnings of genes D and B, respectively. Four minor locations map at 18 to 22, 28 to 32, 50 to 56, and 70 to 74% of the genome. The 70 to 74% site corresponds to the beginning of the A gene. The major locations on 4X174 are at 8 to 10, 50 to 54, and 92 to 94% of the genome. The 50 to 54% site is at the start of the H
more » ... ene and has an equivalent minor site on S13, but it is not a promoter site. Three minor sites on OX174, at 20 to 24, 26 to 32, and 68 to 74% of the genome, correspond to sites on S13. The data confirm the locations of sites identified by restriction fragment binding experiments (E. Rassart and J. H. Spencer, J. Virol. 27:677-687, 1978) and the assignment of putative promoters at the starts of genes A, B, and D. form I (RFI) DNA has been described elsewhere (11). E. coli RNA polymerase was a generous gift of F. Grosveld, and its purification by the method of Bur-179 on May 9, 2020 by guest
doi:10.1128/jvi.29.1.179-184.1979 fatcat:kygcxxhxbfdndb4iptxyjecwmq