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AbstractMorphological and functional optoacoustic imaging is enhanced by dedicated transgene reporters, in analogy to fluorescence methods. The development of optoacoustic reporters using protein engineering and directed evolution would be accelerated by high-throughput in-flow screening for intracellular, genetically encoded, optoacoustic contrast. However, accurate characterization of such contrast is impeded because the optoacoustic signals depend on the cell's size and position in the flowdoi:10.1038/s41598-021-81584-y pmid:33500461 fatcat:r7oetm4wibcpdo3lr5y5w4td2y