Interaction Between Insecticide Resistance-Associated Genes and Malaria Transmission in Anopheles Gambiae S. L. During a Cluster-Randomized Controlled Trial of A "lethal House Lure" in Central Côte D'ivoire [post]

Rosine Z. Wolie, Alphonsine A. Koffi, Ludovic P. Ahoua Alou, Eleanore D. Sternberg, Oulo N'Nan-Alla, Amal Dahounto, Florent H.A. Yapo, Kpahe H.M. Kanh, Soromane Camara, Welbeck A. Oumbouke, Innocent Z. Tia, Prof. Simon-Pierre A. Nguetta (+2 others)
2021 unpublished
Background There is evidence that the Kdr L1014F and Ace-1R G119S mutations involved in pyrethroid and carbamate resistance in Anopheles gambiae influence malaria transmission in sub Saharan Africa. This is likely due to changes in behavior, life history, vectorial competence and capacity. In the present study, performed as part of a two-armed cluster randomized controlled trial (CRT) evaluating the impact of household screening plus a novel insecticide delivery system (In2Care EaveTubes), we
more » ... vestigated the distribution of insecticide target site mutations and their association with the infection status in wild An. gambiae s.l populations. Methods Mosquitoes were captured in 40 villages around Bouaké by human landing catches (HLC), from May 2017 to April 2019. Randomly selected sample of infected and uninfected An.gambiae s.l. with Plasmodium sp. were identified to species and then genotyped for Kdr L1014F and Ace-1R G119S mutations using quantitative polymerase chain reaction (qPCR) assays. The frequencies of the two alleles were compared between An. coluzzii and An. gambiae and then between infected and uninfected groups for each species. Results The presence of An. gambiae (49 %) and An. coluzzii (51%) was confirmed in Bouaké. Both species seemed to transmit equally Plasmodium parasites. Over the study period, the average frequency of the Kdr L1014F and Ace-1R G119S mutations did not vary significantly between study arms. However, the frequency of the Kdr L1014F and Ace-1R G119S resistance alleles were significantly higher in An. gambiae than in An. coluzzii (OR [95%CI]: 59.64 [30.81-131.63] for Kdr and OR [95%CI]: 2.79 [2.17–3.60], for Ace-1R). For both species, there were no significant differences in Kdr L1014F or Ace-1R G119S genotypic and allelic frequency distribution between infected and uninfected specimens (p > 0.05). Conclusions Either alone or in combination, Kdr L1014F and Ace-1R G119S showed no significant association with Plasmodium infection in wild An.gambiae and An. coluzzii, demonstrating similar competence for Plasmodium transmission in Bouaké. Additional factors influencing competence in natural population and those outside allele measurements contributing to resistance should be consider when establishing link between insecticide resistance and vector competence.
doi:10.21203/ fatcat:uvyp4csoufgnjm7f6gejl6rbeu