To estimate the 1axative threshold ( LI)and 50% effective dose( ED5D)for diarrhea of a galactooligosaccharide ( GOS )syrup ( 43% GOS ;95 % 1actose ; 18% glucose ; 6. 3% galactose ;24 % water ) , we asked 24 healthy subjects ( 12men and 12women ;age , 20 to 58 years ; weight , 39. O to 96 . O kg)to ingest 190mL of aqueous solutions containing 5 different amounts ( Il. 7, 23. 3, 46 . 7, 70 . 0, 93. 3g)of the syrup once every week from a low content to high one . Of the 24 s 呵 ects , 16 had

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... a after ingestion of any test beverage . We estimeted the LT and ED50 of the GOS syrup by using a regression equation between the minimal dose levels ( glkg body weght )of the syrup caused diarrhea and the cumulative incidence of diarrhea . For the entire population , the estimated LT was O, 68 gtkg( 0. 30 g 川 ( g as GOS )and 匡 he ED50 was 1, 3991kg ( O. 60 glkg as GOS ) . For men , the LT and ED50 were O . 65 g / kg and 1. 13gn ( g, respectively , as compared with O . 9091kg and 1. 50 gtkg for women . An increased frequency of bowel movements seemed to increase the incidence of diarrhea , The other abdominal symptoms reported were borborygmus ( 88% ) , flatus ( 58 % ) , and abdominal distention ( 46% ) , alI of which we τe transient , as was diarrhea . We also examined the safety of ingesting daily near amount of LT of the GOS syrup ( 15 帥 erson daily as GOS ) for 2 weeks in 20 hea脆hy adults . Although this dose increased the occurrence of abdominal gas in these subjects , the prevalence of diarrhea was un − changed as compared with that for the placebo beverage . These results show that the GOS syrup is safe even in repeated intake for near amount of LT , '