Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS

Ying Chen, Olga Berejnaia, Jinqi Liu, Sheng-Ping Wang, Natalie A. Daurio, Wu Yin, Rafael Mayoral, Aleksandr Petrov, Takhar Kasumov, Guo-Fang Zhang, Stephen F. Previs, David E. Kelley (+1 others)
2018 American Journal of Physiology. Endocrinology and Metabolism  
DG. Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS. Numerous studies have implicated dyslipidemia as a key factor in mediating insulin resistance. Ceramides have received special attention since their levels are inversely associated with normal insulin signaling and positively associated with factors that are involved in cardiometabolic disease. Despite the growing literature surrounding ceramide biology, there are limited data regarding the activity of ceramide
more » ... ctivity of ceramide synthesis and turnover in vivo. Herein, we demonstrate the ability to measure ceramide kinetics by coupling the administration of [ 2 H]water with LC-MS/MS analyses. As a "proof-of-concept" we determined the effect of a diet-induced alteration on ceramide flux; studies also examined the effect of myriocin (a known inhibitor of serine palmitoyltransferase, the first step in sphingosine biosynthesis). Our data suggest that one can estimate ceramide synthesis and draw conclusions regarding the source of fatty acids; we discuss caveats in regards to method development in this area.
doi:10.1152/ajpendo.00457.2017 pmid:29509438 fatcat:nj4z3jm7kngtfn5othcei3yekq