Curcumin-induced suppression of adipogenic differentiation is accompanied by activation of Wnt/β-catenin signaling

Jiyun Ahn, Hyunjung Lee, Suna Kim, Taeyoul Ha
2010 American Journal of Physiology - Cell Physiology  
Curcumin, a polyphenol found in the rhizomes of Curcuma longa, improves obesityassociated inflammation and diabetes in obese mice. Curcumin also suppresses adipocyte differentiation, although the underlying mechanism remains unclear. Here, we used 3T3-L1 cells to investigate the details of the mechanism underlying the anti-adipogenic effects of curcumin. Curcumin inhibited mitogen-activated protein kinase (MAPK) (ERK, JNK, and p38) phosphorylation that was associated with differentiation of
more » ... L1 cells into adipocytes. During differentiation, curcumin also restored nuclear translocation of the integral Wnt signaling component ␤-catenin in a dose-dependent manner. In parallel, curcumin reduced differentiation-stimulated expression of CK1␣, GSK-3␤, and Axin, components of the destruction complex targeting ␤-catenin. Accordingly, quantitative PCR analysis revealed that curcumin inhibited the mRNA expression of AP2 (mature adipocyte marker) and increased the mRNA expression of Wnt10b, Fz2 (Wnt direct receptor), and LRP5 (Wnt coreceptor). Curcumin also increased mRNA levels of c-Myc and cyclin D1, well-known Wnt targets. These results suggest that the Wnt signaling pathway participates in curcumin-induced suppression of adipogenesis in 3T3-L1 cells.
doi:10.1152/ajpcell.00369.2009 pmid:20357182 fatcat:g7mbgtr3cvf6bjwea56iq5mjnm