A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is
Equilibrium constants for the adenylylation of T4 DNA ligase have been measured at 10 pH values. The values, when plotted against pH, fit a titration curve corresponding to a pK a of 8.4 ؎ 0.1. The simplest interpretation is that the apparent pK a is that of the 6-amino group of the AMP-accepting residue Lys 159 . Based on the pH dependence of the equilibrium constants, the value at pH 7.0 is 0.0213 at 25°C, corresponding to ⌬G o ؍ ؉2.3 kcal mol ؊1 . From this value and the standard freedoi:10.1074/jbc.274.13.8586 pmid:10085093 fatcat:36gijjlewjbjjfruuekm66xyam