Cellular and Molecular Organization of the Rhesus Macaque Dorsal Horn with a Comparison to Mouse [article]

Cynthia Arokiaraj, Michael Kleyman, Alexander Chamessian, Stephanie Shiers, Byungsoo Kang, Meaghan Kennedy, Yitao Chen, Richard Dum, Ryan Patterson, David Lewis, Yawar Qadri, Ariel Levine (+3 others)
2022 bioRxiv   pre-print
The spinal dorsal horn transforms incoming somatosensory information and transmits it supraspinally to generate modality-specific sensory percepts. The lack of an established framework for the molecular and cellular organization of the dorsal horn across species has greatly hampered delineating precisely how this region processes somatosensory information, including pain. Furthermore, the translation potential of the rodent work is unclear without data from higher order species. To fill these
more » ... ps, we performed single nucleus RNA-sequencing of Rhesus macaque dorsal horn and compared the results to a recently reported meta-analysis of mouse. Because dorsal horn laminae serve as a key organizing principle for function, we also determined the laminar location of each identified cell type. The work provides a comprehensive cross-species cellular and molecular database that will be critical for decoding the logic of dorsal horn somatosensory circuits and validating preclinical targets.
doi:10.1101/2022.04.01.486135 fatcat:rqmwk442kjgrhflwgzbytexzeq