Visualization of Quantum Dots by Freeze-fracture Electron Microscopy

B Papahadjopoulos-Sternberg, KC Weng, JW Park
2006 Microscopy and Microanalysis  
Semiconductor quantum dots (QDs) are a new class of promising fluorescent probes for many biological and biomedical applications due to their advantages over conventional dyes [1] . They exhibit excellent photostability and their emission spectra are narrow, symmetrical, and tunable according to their size and material composition, allowing closer spacing of different probes without spectral overlap. Independently, tremendous effort has been dedicated to design and optimize nanoscale targeted
more » ... anoscale targeted drug delivery systems (tDDS) including target-specific liposomal therapeutics displaying remarkable anti-tumor efficacy and relatively mild toxicity profiles [2,3]. Our aim is to combine both fruitful developments by incorporation of QDs into immunoliposome nanocomplexes to develop novel molecular imaging agents for applications in cancer diagnosis and treatment. Freeze-fracture electron microscopy (ff-em) is a powerful technique to characterize nanometer-and micrometer-size drug/gene carriers including tDDS [4] [5] [6] [7] [8] [9] [10] . Using ff-em we visualized carboxyl QDs, naked and complexed with liposomes as well as with HER-2-tageting Ils.
doi:10.1017/s1431927606066335 fatcat:4gaim5csprexvodaeqpzqt7ulu