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ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM SOIL ABLE TO LIVE ON LIGNIN AS ACARBON SOURCE AND TO PRODUCE ENZYMES WHICH CLEAVE THE β-O-4 BOND IN A LIGNIN MODEL COMPOUND

R Bi, O Spadiut, M Lawoko, H Brumer, G Henriksson
2012 CELLULOSE CHEMISTRY AND TECHNOLOGY Cellulose Chem. Technol   unpublished

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Several strains of fungi were isolated and identified from Scandinavian soil using agar plates with lignin as a carbon source. The strains grew significantly faster on this medium than on control plates without lignin. Different types of technical lignins were used, some of which contained trace amounts of sugars, even if the increased growth rate seemed not related to the sugar content. Some strains were cultivated in shaking flask cultures with lignin as a carbon source, with lignin
more » ... consumed by microbes-while accumulation of the microorganism biomass occurred. The cell-free filtrates of these cultures could reduce the apparent molecular weights of lignosulphonates, while the culture filtrate of one strain could cleave the β-O-4 bond in a lignin model compound.
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R Bi, O Spadiut, M Lawoko, H Brumer, G Henriksson. "ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM SOIL ABLE TO LIVE ON LIGNIN AS ACARBON SOURCE AND TO PRODUCE ENZYMES WHICH CLEAVE THE β-O-4 BOND IN A LIGNIN MODEL COMPOUND." CELLULOSE CHEMISTRY AND TECHNOLOGY Cellulose Chem. Technol 46.3-4 (2012)