A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture
Journal of the Royal Society Interface
A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture. Abstract Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods utilize glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for
... collagen Type I covalently on PDMS utilizing ascorbic acid (AA) as a crosslinker instead of GA. We compare the novel method against physisorption and GA crosslinker based methods. The coatings are characterized by immunostaining, contact angle measurement, atomic force microscopy and infrared spectroscopy, and evaluated in static and stretched human adipose stem cell (hASC) cultures up to 13 days. We found that AA can replace GA as a crosslinker in the covalent coating method and that the coating is durable after sonication and after six days of stretching. Furthermore, we show that hASCs attach and proliferate better on AA crosslinked samples compared to physisorbed or GA based methods. Thus, in this paper we provide a new PDMS coating method for studying cells, such as hASCs, in static and dynamic conditions. The proposed method is an important step in the development of PDMS based devices in cell and tissue engineering applications.