Physiological and Clastogenic Effects of SDS in Certain Allium Species of Nagaland

Thejasenuo Kirha, Sanjay Kumar
unpublished
The tremendous use of the chemical in daily life activity, animal and plant system prompted us to determine the physiological as well as clastogenic effects of this chemical in plant genetic system. The different Allium species (A. hookeri, A.tuberosum, A.chinense, A.ascalonicum) and concentrations (500 ppm,1000 ppm and 3000 ppm) for 3 h was used in the present study to observe the said effects on the genetics of this species. SDS (sodium dodecyl sulphate) is generally used in cosmetics,
more » ... n cosmetics, shampoos, soaps, detergents, as a leather softening agent in industry, as flocculating and de-inking agent in paper industry and engine degreasers, importantly used in nasal and ocular drug delivery, in trans-epidermal, to boost intestinal absorption of drugs in animal models and in biochemical research in the laboratories. The Methodology includes the young buds of different Allium species collected from the field and maintained at a room temperature of 23 ± 2°C for control and treatment study Flower buds for meiotic study were fixed in carnoys solution (ethyl alcohol and glacial acetic acid 3:1) for 24 hrs and followed by 70% ethyl alcohol. The buds were treated at different concentration for 3 h. The maximum physiological and clastogenic aberrations observed was stickiness, laggards, abnormal metaphase, micronuclei and ring chromosomes in the studied Allium species. Although, the maximum abnormality observed as stickiness and laggards (physiological) and micronuclei (clastogenic aberration) in the Allium species indicates the toxicity of SDS on the genomes of the plants.
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