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IgG Response to the Immunoreactive Region of Parvovirus B19 Nonstructural Protein by Immunoblot Assay with a Recombinant Antigen

Simona Venturoli, Giorgio Gallinella, Elisabetta Manaresi, Giovanna Gentilomi, Monica Musiani, Marialuisa Zerbini
1998 Journal of Infectious Diseases  

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https://web.archive.org/web/20180726155433/https://watermark.silverchair.com/178-6-1826.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAZ8wggGbBgkqhkiG9w0BBwagggGMMIIBiAIBADCCAYEGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMElZgKAYnlAo5CSVtAgEQgIIBUg7YdVlFqlbvqF5PerQxjD3MKD0j4QfCAy5Y6hyRFLhu9_gEjvivYx_IQh50QDkBXQ6uNwU22_As0FZOjnS_dSaEb-SunS8dmvTbMewlffs7ZR39jLF6olxz-AHu-4xi8OMY2CQZoVMIB0PNTwSsTrNhqr8R3riYuf9o2Kv88opsLR6n2ADuD6wDgCEPj2Cs6Ydp-dGxOs-c6AuARt1se6-iduX6Rp75dwHIyE69nwnosrWhIC206nLEAnDelNHF4TfFMUV_-dXYNM0dTJZE7kz8sfelBqeM3R3WT0uDzq0A62kDoG2PBORObSL21SaA0Zv8NeGJp0jDpxPR5o39Klnev-EWGOOqqvgIyE01rDIj5U6lcPfoz4zOywRrOPU9sYLPut2PyQBMFQoZa9g_pn1OcjJyQ_zf6zMCcmLDZ6IkyVM5-Kv5Y2rKUHmIG_d7yU8E

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A total of 190 serum samples from patients with different clinical manifestations in whom B19 parvovirus infection was suspected and 108 control serum samples were analyzed for the IgG response against parvovirus B19 nonstructural protein. An immunoblot assay was developed using the immunoreactive region of the B19 nonstructural protein as recombinant antigen. Virologic (B19 DNA) and serologic (IgM and IgG anti-VP) markers of B19 infection were also investigated. There was an even distribution
more » ... f IgG nonstructural antibodies in serum samples of parvovirus B19-infected patients and controls (27.7% and 21.7%, respectively) that were mainly associated with patterns of past B19 infection.
doi:10.1086/314500 pmid:9815244 fatcat:wtynkgtxqbeufetvpyvhgn5qcm
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Simona Venturoli, Giorgio Gallinella, Elisabetta Manaresi, Giovanna Gentilomi, Monica Musiani, Marialuisa Zerbini. "IgG Response to the Immunoreactive Region of Parvovirus B19 Nonstructural Protein by Immunoblot Assay with a Recombinant Antigen." Journal of Infectious Diseases 178.6 (1998) 1826-1829