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In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular space (referred to as R b ) and (II) the basal adhesion of the endothelial cells (α, alpha). Physiologically, both parameters are very important fordoi:10.3390/bios8030064 pmid:29973526 fatcat:6pesmqojdfebvosbpyxkfzxwka