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Limitations of alignment-free tools in total RNA-seq quantification
2018
BMC Genomics
Alignment-free RNA quantification tools have significantly increased the speed of RNA-seq analysis. However, it is unclear whether these state-of-the-art RNA-seq analysis pipelines can quantify small RNAs as accurately as they do with long RNAs in the context of total RNA quantification. Result: We comprehensively tested and compared four RNA-seq pipelines for accuracy of gene quantification and fold-change estimation. We used a novel total RNA benchmarking dataset in which small non-coding
doi:10.1186/s12864-018-4869-5
pmid:29969991
pmcid:PMC6042521
fatcat:k5bjud7pz5dybh7ntsiwn2ewfq