CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

Ian Dobbie, Michael Phillips, Maria Harkiolaki, David Susano Pinto, Richard Parton, Ana Palanca, Manuel Garcia-Moreno, Ilias Kounatidis, John Sedat, David Stuart, Alfredo Castello, Martin Booth (+1 others)
2020 Optica  
Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from
more » ... tly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.
doi:10.1364/optica.393203 pmid:34277893 pmcid:PMC8262592 fatcat:2rv4xeo2lbcsjckjq3esgd33xe