Phytochemical Screening and Hypoglycaemic Effect of Methanol Extract of Solanum anomalum Thonn. Fruits in Alloxan induced Hyperglycaemic and Normal Wistar Rats

Abubakar Bilyaminu, Kwanashie Helen, Zezi Abdulkadir Umar
2016 International Journal of Current Research in Biosciences and Plant Biology  
The antidiabetic potential of crude ethanolic extract (452 and 678mg/kg), aqueous fraction(452mg/kg) and N-hexane fraction(452mg/kg) of the fruits of Solanum anomalum was evaluated on Alloxan-induced diabetic Rats and the results compared to the standard drug, Glibenclamide(2.5mg/kg). Animals were divided into six (6) groups of six Rats each. Pretreatment with Alloxan was carried out with 150mg/kg and animals with blood glucose levels (BGL) above 200mg/dl in 72 hours were considered diabetic.
more » ... udy was carried out in acute case (upto 6 hours) and in prolonged treatment (14 days). Results show a significant (p<0.001) reduction in blood glucose level of the diabetic rats in both acute and prolonged treatment comparable to Glibenclamide. The LD50 of the extract was determined to be 2260 + 131.78mg/kg using the method of Tainter and Miller. Phytochemical analyses of the fruit show the presence of Alkaloids, Saponins, Cardiac glycosides, Terpenes, Flavonoids and Tanins. Ethanolic extract and fractions of the fruits of Solanum anomalum were therefore found to possess significant antidiabetic effect.
doi:10.20546/ijcrbp.2016.310.021 fatcat:a524j4eryrcb7gdbchec4wkyue